The research of our group focuses on the study of the mechanisms involved in the resistance of plants to biotic agents (viroids, viruses, bacteria, fungi) using biochemical, molecular and metabolomic approaches, with the aim of learning the function of genes, proteins and metabolites involved in the plant's response to attack by different pathogens.
1.- Metabolomic analysis by gas chromatography and mass spectrometry of the defensive response of leaves of tomato plants 'Rio Grande' Pto infected with two strains of the bacterial pathogen Pseudomonas syringæ pv. tomato DC3000 carrying (incompatible interaction) or not (compatible interaction) the AvrPto gene has shown significant differences in the emission of volatile organic compounds (VOCs) by the tomato leaves. The comparison of the metabolomic profiles between plants subjected to both types of infection revealed that different volatile compounds were produced in each interaction. It was observed that the incompatible interaction, showing a total resistance to Pseudomonas, was associated with the production of (Z)-3-hexenol esters, such as (Z)-3-hexenyl acetate and (Z)-3-hexenyl butanoate, as well as some hydroxylated monoterpenes, including α-terpineol, 4-terpineol and linalool in the infected leaves. On the other hand, emission of methyl salicylate and monoterpenes such as limonene and α-pinene occurred in the compatible interaction, in which tomato leaves showed strong symptoms of the bacterial disease (López-Gresa et al. 2017). In addition, we have found that some of these VOCs display in vitro antibiotic activity against Pseudomonas. Moreover, exogenous treatments of tomato plants with these compounds provoked the induction of defensive genes, the stomatal closure and increased resistance of tomato plants to Pseudomonas infection (López-Gresa et al. 2018). These results suggest that these VOCs could be a dual role in tomato plants: as protective molecules and as defence inducers. In addition, because their chemical nature, they could also be implicated as playing a role in interplant communication through the atmosphere. We have filed a patent (PCT/ES2018/070) on the usage and the application method for (Z)-3-hexenyl butanoate (HB) as an inducer of stomata closure in order to protect plants against pathogen attack and drought. In addition to this biological role, our latest results (Payá et al. 2020) show that the exogenous application of HB in grapevine (Vitis vinifera) accelerates ripening.
2.- We have demonstrated the induction of the expression of different translation factors and ribosomal proteins as a consequence of Citrus Exocortis Viroid (CEVd) infection in tomato. This result indicates that a non-coding pathogen cause changes in the transcriptional machinery. In particular, the elongation factors 1 and 2 (eEF1A and eEF2) and the initiation factor of the translation 5-alpha (eIF5A) were identified as induced by CEVd, and a reproducible interaction between eEF1A and the viroid was demonstrated as well (Lisón et al. 2013). Recently, we have demostrated that CEVd causes ribosomal stress in tomato plants (Cottilli et al. 2019). Our latest results (Vazquez et al. 2020) using ethylene-insensitive Never ripe (Nr) tomato mutants suggest an important role of this phythormone in the ribosomal stress caused by viroidal infection.
3.- We have demonstrated the role of flavonoids and Hydroxycinnamic Acid Amide (HCAAs) in tomato defence against virus (Campos et al. 2019) and Pseudomonas syringae DC3000 (Zacarés et al. 2007, López-Gresa 2011, Campos et al. 2014), respectively. We have identified new compounds of phenolic nature whose synthesis is induced in both compatible and incompatible tomato-pathogen interactions. One of these metabolites, the trans-feruloylnoradrenaline (t-FNA), is a novel compound with a powerful antioxidant activity (International Patent WO2011ES70269 20110415). Tomato transgenic plants overexpressing THT, a gene involved in the HCAAs biosynthesis, displayed enhanced resistance to Pseudomonas syringae DC3000.
4.- We have obtained solid evidence that gentisic acid (GA), a metabolic derivative of salicylic acid (SA), acts as a complementary signal additional to salicylic acid for the activation of tomato plant defences against pathogens (Bellés et al. 1999). We have also found that SA and GA could have a role as signal molecules involved in the silencing of RNA against viruses and viroids (Campos et al. 2014, López-Gresa et al. 2016). Recently, the defensive role of GABA in the CEVd-tomato interaction has been described (López-Gresa et al. 2019).
Other Group Members
Temporary VisitorsTFM: Julia Pérez, Paula Galarza TFG: Magdalena Escánez
Payá C, López Gresa MP, Intrigliolo DS, Rodrigo I, Bellés JM, Lisón P (2020)(Z)-3-Hexenyl Butyrate induces stomata closure and ripening in Vitis viniferaAgronomy 10(8): 1122
Ibáñez MD, López-Gresa MP, Lisón P, Rodrigo I, Bellés JM, González-Mas MC, Blázquez (2020)Essential oils as natural antimicrobial and antioxidant products in the Agrifood IndustryNereis 12: 55-69
Vázquez-Prol F, López-Gresa MP, Rodrigo I, Bellés JM, Lisón P (2020)Ethylene is involved in symptom development and ribosomal stress of tomato plants upon Citrus Exocortis Viroid infectionPlants 9 (5): 582
González S, LLinares JV, Al Hassan M, Fita A, Collado F, Lisón P, Vicente O, Boscaiu M (2020)Physiological and morphological characterisation of Limonium species in their natural habitats: insights into their abiotic stress responsesPlant and Soil 449: 267 - 284
López Gresa MP; Payá C, Bellés JM, Rodrigo I, Lisón P (2019)Aroma del tomate para proteger cultivosApte Techno. pp. 12 - 12
Patrick Cottilli, Borja Belda-Palazón, Charith Raj Adkar-Purushothama, Jean-Pierre Perreault, Enrico Schleiff, Ismael Rodrigo, Alejandro Ferrando, Purificación Lisón (2019)Citrus exocortis viroid causes ribosomal stress in tomato plantsNucleic Acids Research, 47(16): 8649–8661
Solé-Gil A, Hernández-García J, López-Gresa MP, Blázquez MA, Agustí J (2019)Conservation of thermospermine synthase activity in vascular and non-vascular plantsFrontiers in Plant Science 10: 10.3389/fpls.2019.00663
: López-Gresa MP, Payá C, Rodrigo I, Bellés JM, Barceló S, Choi YH, Verpoorte R, Lisón P (2019)Effect of Benzothiadiazole on the metabolome of tomato plants infected by Citrus Exocortis ViroidViruses 11: 437-452
Bellés JM, Lisón P, López-Gresa MP, Payá C, Rodrigo I (2019)El aroma de la resistencia de las plantas a las enfermedades. Tierras de Castilla y LeónAgricultura. pp. 34- 38
González-Orenga S, Ferrer-Gallego PP, Laguna E, López-Gresa MP, Donat-Torres MP, Verdeguer M, Vicente O, Boscaiu M. (2019)Insights on Salt Tolerance of Two Endemic Limonium Species from SpainMetabolites 9: 294-316