ID pYFC43 standard; circular DNA; ; 12047 BP. DE pMDC24. KW pMDC24. CC This file is created by Vector NTI CC http://www.informaxinc.com/ CC ORIGDB|GenBank CC VNTDATE|269952104| CC VNTDBDATE|423419386| CC LSOWNER| CC VNTAUTHORNAME|Alejandro| FH Key Location/Qualifiers FH FT CDS complement(8427..9221) FT /vntifkey="4" FT /label=kanamycin\resistance FT misc_feature complement(3387..3412) FT /vntifkey="21" FT /label=RB FT misc_feature 9646..9671 FT /vntifkey="21" FT /label=LB FT CDS complement(9962..10987) FT /vntifkey="4" FT /label=hygromycin\resistance FT rep_origin complement(6046..7046) FT /vntifkey="33" FT /label=pVS1 FT rep_origin complement(7856..8136) FT /vntifkey="33" FT /label=pBR322\origin FT promoter complement(2359..3127) FT /vntifkey="29" FT /label=2X35S\promoter FT terminator 6..263 FT /vntifkey="43" FT /label=nos\terminator FT repeat_region 319..443 FT /vntifkey="34" FT /label=attR2 FT CDS complement(484..789) FT /vntifkey="4" FT /label=ccdB FT CDS complement(1131..1790) FT /vntifkey="4" FT /label=CMr FT repeat_region 1899..2028 FT /vntifkey="34" FT /label=attR1 FT misc_feature complement(2042..2093) FT /vntifkey="21" FT /label=FlexibleLinker FT misc_feature complement(2094..>2350) FT /vntifkey="21" FT /label=YFC SQ Sequence 12047 BP; 2864 A; 3207 C; 3106 G; 2870 t; aattcagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt 60 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca 120 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt 180 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa 240 tgtttgaacg atcggggaaa ttcgagctcc accgcggtgg gcggccgctc tagaactagt 300 taattaagga attatcgaac cactttgtac aagaaagctg aacgagaaac gtaaaatgat 360 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa 420 acacaacata tccagtcact atggtcgacc tgcagactgg ctgtgtataa gggagcctga 480 catttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct 540 gagatcagcc acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat 600 catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg agactttatc 660 tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat 720 atcactctgt acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt 780 aaactgcatt tcaccagtcc ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc 840 gggcgacctc agccatccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac 900 gggcttcatt ctgcatggtt gtgcttacca gaccggagat attgacatca tatatgcctt 960 gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcacacct 1020 ctttttgaca tacttcgggt atacatatca gtatatattc ttataccgca aaaatcagcg 1080 cgcaaatacg catactgtta tctggctttt agtaagccgg atcctctaga ttacgccccg 1140 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca 1200 tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta 1260 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa 1320 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac 1380 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt 1440 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc 1500 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc 1560 attgccatac ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc 1620 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga 1680 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga 1740 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc 1800 ttagctcctg aaaatctcgc cggatcctaa ctcaaaatcc acacattata cgagccggaa 1860 gcataaagtg taaagcctgg ggtgcctaat gcggccgcca tagtgactgg atatgttgtg 1920 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta 1980 tatcatttta cgtttctcgt tcagcttttt tgtacaaact tgtttgatag cttggcgcgc 2040 caagagatcc acctccacca gatccacctc caccagatcc acctccaccg gccttgtaca 2100 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat 2160 cgcgcttctc gttggggtct ttgctcaggg cggactggta gctcaggtag tggttgtcgg 2220 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca 2280 cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct 2340 tgtcggccat ggtacccggg gatcctctag agtcgaggtc ctctccaaat gaaatgaact 2400 tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc 2460 agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc 2520 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttc 2580 aacgatggcc tttcctttat cgcaatgatg gcatttgtag gagccacctt ccttttccac 2640 tatcttcaca ataaagtgac agatagctgg gcaatggaat ccgaggaggt ttccggatat 2700 taccctttgt tgaaaagtct caattgccct ttggtcttct gagactgtat ctttgatatt 2760 tttggagtag acaagtgtgt cgtgctccac catgttatca catcaatcca cttgctttga 2820 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt 2880 gggaccactg tcggcagagg catcttcaac gatggccttt cctttatcgc aatgatggca 2940 tttgtaggag ccaccttcct tttccactat cttcacaata aagtgacaga tagctgggca 3000 atggaatccg aggaggtttc cggatattac cctttgttga aaagtctcaa ttgccctttg 3060 gtcttctgag actgtatctt tgatattttt ggagtagaca agtgtgtcgt gctccaccat 3120 gttgacctgc aggcacgcca agcttggcac tggccgtcgt tttacaacgt cgtgactggg 3180 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc 3240 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg 3300 aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat 3360 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata 3420 acggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca 3480 accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc 3540 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta 3600 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag 3660 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc 3720 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca 3780 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac 3840 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt 3900 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga 3960 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac 4020 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc 4080 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt 4140 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca 4200 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta 4260 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt 4320 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga 4380 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag 4440 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc 4500 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc 4560 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa 4620 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc 4680 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga 4740 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg 4800 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa 4860 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg 4920 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg 4980 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg 5040 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct 5100 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg 5160 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca 5220 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg 5280 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa 5340 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc 5400 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc 5460 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga 5520 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta 5580 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg 5640 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg 5700 tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc 5760 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg 5820 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg 5880 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg 5940 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc 6000 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg 6060 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag 6120 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg 6180 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc 6240 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga 6300 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg 6360 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg 6420 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag 6480 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt 6540 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc 6600 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc 6660 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac 6720 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc 6780 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta 6840 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg 6900 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag 6960 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat 7020 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca 7080 taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg 7140 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg 7200 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac 7260 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct 7320 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 7380 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca 7440 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta 7500 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 7560 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 7620 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 7680 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 7740 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 7800 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 7860 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 7920 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 7980 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 8040 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 8100 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 8160 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 8220 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 8280 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 8340 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 8400 gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt 8460 attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct 8520 tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc 8580 ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg 8640 ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa 8700 aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc 8760 acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta 8820 ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac 8880 agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca 8940 tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt 9000 ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag 9060 gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc 9120 agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg 9180 atcagttttt tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt 9240 ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac 9300 tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa 9360 agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag 9420 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa 9480 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg 9540 ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg 9600 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg 9660 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta 9720 ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag 9780 gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact aagggtttct 9840 tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta 9900 ctcacacatt attatggaga aactcgagct tgtcgatcga cagatccggt cggcatctac 9960 tctatttctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt 10020 ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag 10080 tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga 10140 aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc 10200 ggagtcgtgg cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct 10260 ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc 10320 cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat 10380 tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa 10440 gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt 10500 gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt 10560 gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag 10620 cgatcgcatc catagcctcc gcgaccggtt gtagaacagc gggcagttcg gtttcaggca 10680 ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctaa 10740 actccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa 10800 cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc 10860 ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg 10920 agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct 10980 ttttcatatc tcattgcccc ccgggatctg cgaaagctcg agagagatag atttgtagag 11040 agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa 11100 ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc 11160 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg 11220 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt 11280 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt 11340 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag 11400 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag 11460 tgtcgtgctc caccatgtta tcacatcaat ccacttgctt tgaagacgtg gttggaacgt 11520 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag 11580 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt 11640 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt 11700 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat 11760 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta 11820 gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 11880 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac 11940 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt 12000 gagcggataa caatttcaca caggaaacag ctatgaccat gattacg 12047 //