My research interests include the molecular genetic analysis of the hormonal regulation of plant development. Much of the focus of our work has been on the control of reproductive organs by gibberellins, as well as plant architecture.
Tools and approaches used in our work include: cloning genes controlling hormone gibberellin metabolism, studying their function using reverse genetics (over-expression or silencing in transgenic plants of tomato and tobacco) and recently, as a result of the acquisition of a modern equipment of mass spectroscopy in the IBMCP, we could also incorporate the analysis and quantification of major plant hormones among the methods used in our research.
Our current research is directed towards understanding the role of gibberellins in ovules formation (grant nº BIO2014-55946) and the role of plant hormones in the induction of in vitro embryogenesis (grant nº AGL2014-55177-R).
In addition to the projects described above, I am scientific supervisor of a platform for Plant Hormones Analysis at the IBMCP capable of quantifying 20 hormonal compounds (GAs, IAA, ABA, JA, SA and CKS) in a variety of plant materials at concentrations below 0.1ng/g. Because the analytical methods for determining hormone levels in plant tissues are essential for elucidating the role and function of plant hormones in growth and development, we have been involved in collaborative projects with groups from different countries interested in the hormonal regulation of different developmental responses.
Flowering in Tobacco Needs Gibberellins but is not Promoted by the Levels of Active GA1 and GA4 in the Apical Shoot. Gallego-Giraldo L., García-Martínez J. L., Moritz T., and López-Díaz I. Plant Cell Physiol. 48: 615-625 (2007).
Gibberellin Homeostasis in Tobacco is Regulated by Metabolism Genes with Different Gibberellin Sensitivity. Gallego-Giraldo L., Ubeda-Tomas S., Gisbert C., García-Martínez J. L., Moritz T., and López-Díaz I. Plant Cell Physiol. 49, No. 5: 679-690 (2008).
Silencing C19-GA 2-oxidases induces parthenocarpic development and inhibits lateral branching in tomato plants. Liliam Martinez-Bello; Thomas Moritz; Isabel Lopez-Diaz. J. Exp. Bot. 66 (19): 5897-5910 (2015)
The gibberellin precursor GA12 acts as a long-distance growth signal in Arabidopsis. Regnault T, Davière J, Wild M, Sakvarelidze-Achard L, Heintz D, Carrera Bergua E, Lopez Diaz I, Gong F, Hedden P, Achard P. Nat Plants. 1(6):15073 (2015).
Increased Nicotiana tabacum fitness through positive regulation of carotenoid, gibberellin and chlorophyll pathways promoted by Daucus carota lycopene beta-cyclase (Dclcyb1) expression. Moreno JC, Cerda A, Simpson K, Lopez-Diaz I, Carrera E, Handford M, Stange C. Journal of Experimental Botany 67: 2325-2338, (2016).
Tissue-specific regulation of gibberellin signaling fine-tunes Arabidopsis iron deficiency responses. Davière J, Carrera Bergua E, Lopez Diaz I, and Achard P . Dev Cell. 18;37(2):190-200, (2016).
Salt Induces Features of a Dormancy-Like State in Seeds of Eutrema (Thellungiella) salsugineum, a Halophytic Relative ofArabidopsis. Yana Kazachkova, Asif Khan, Tania Acuña, Isabel López-Díaz, Esther Carrera, Inna Khozin-Goldberg, Aaron Fait and Simon Barak. Front. Plant Sci.7: p1071 03 August (2016).. DOI=10.3389/fpls.2016.01071