Research groups

Grupo: Abscisic acid signaling

Abscisic acid (ABA) plays a crucial role to integrate plant response to abiotic stress (particularly drought and salinity) into the regulation of plant growth and development. An increase in ABA levels and the subsequent plant response to the hormone are key components of the adaptive mechanism to resist/avoid those forms of abiotic stress. Accordingly, the characterization of ABA signaling offers a high biotechnological potential to improve plant tolerance to drought and salinity. 

Because of its essential function in plant stress physiology, elucidating the abscisic acid (ABA) signalling pathway holds enormous promise for application in agriculture. A breakthrough in ABA signaling occurred in 2009, i.e. the discovery of the 14-member PYR/PYL/RCAR family of ABA-receptors (Park et al., 2009; Ma et al., 2009; Santiago et al., 2009). Control of ABA signalling by PYR/PYL/RCAR ABA-receptors involves direct ABA-dependent inhibition of clade A phosphatases type-2C (PP2Cs), for instance ABI1, HAB1, PP2CA, which are key negative regulators of the pathway (Saez et al., 2006; Rubio et al., 2009). Inhibition of PP2Cs leads to activation of sucrose non-fermenting 1-related subfamily 2 (SnRK2) kinases, which regulate stomatal aperture and transcriptional response to ABA. Thus, a core signalling network for ABA has emerged from these findings (Fujii et al., 2009; Cutler et al., 2010).            

Molecular genetics: The ABA signaling group has played a key role in the discovery and characterization of the PYR/PYL/RCAR family of ABA receptors, and their connection with PP2Cs and SnRK2s (Rodriguez et al., 1998; Gonzalez-Guzman et al., 2002; Saez et al., 2004, 2006, 2008; Park et al., 2009; Santiago et al., 2009a, 2009b; Rubio et al., 2009; Vlad et al., 2009; Fujii et al., 2009; Cutler et al., 2010; Vlad et al., 2010; Dupeux et al., 2011a, 2011b; Antoni et al. 2012; Gonzalez-Guzman et al., 2012; Antoni et al., 2013; Merilo et al., 2013; Pizzio et al., 2013). Some of these works are landmarks in ABA signaling and together with results obtained by other research groups have brought about a breakthrough in our knowledge of the pathway. Later on, we have provided insight on the subcellular location of ABA receptors and we have discovered that C2-domain abscisic acid-related proteins mediate the interaction of PYR/PYL/RCAR receptors with the plasma membrane (Rodriguez et al., 2014; Diaz et al., 2016). Concerning ABA-induced transcriptional regulation, we have discovered a link between SWI/SNF chromatin remodeling complexes and core components of ABA signaling (Saez et al., 2008; Han et al., 2012; Peirats-Llobet et al., 2016). We have contributed several genetic strategies that enhance ABA signaling as a valuable tool for improving plant water use. Among them, the constitutive inactivation of PP2Cs (Saez et al., 2006), overexpression of monomeric ABA receptors (Santiago et al., 2009a; Gonzalez-Guzman et al., 2014) and the generation of mutated ABA receptors that enhance ABA-dependent inhibition of PP2Cs (Pizzio et al., 2013). As a result, three patents were filled to cover these findings. More recently, we have played a pioneering role in studies that address the turnover of core ABA signaling components, particularly ABA receptors and PP2Cs (Bueso et al., 2014; Irigoyen et al., 2014; Belda-Palazón et al., 2016; Wu et al., 2016).  We have studied mechanisms that affect subcellular localization and half-life of ABA receptors. As a result we have uncovered a novel route for endosomal degradation of ABA receptors through the ESCRT pathway (Belda-Palazón et al., 2016; Yu et al., 2016) and we have identified a novel family of E3 ligases that mediate the turnover of PP2Cs (Wu et al., 2016). These articles witness our progress in the physiological and biochemical characterization of the ABA signaling pathway.

Agrochemicals: Crystal structures are available for ABA receptors and receptor-ABA-phosphatase complexes, which reveal key details on the mode of interaction between the receptor, the hormone and the protein phosphatase as well as the mechanism of activation of ABA signaling. This information will be used in the structure-assisted identification of synthetic molecules able to act as agonists of ABA receptors and activate ABA signaling in plants. These molecules might have the potential to improve the yield of crop plants under drought stress or any other properties modulated by the ABA pathway in crop or ornamental plants. To this end we will clone and produce recombinant ABA receptors in tomato, orange, grapevine and monocots as targets for screening small molecules capable of acting as agrochemicals through activation of ABA receptors.

 

Staff Researchers

Other Group Members

  • Belda-Palazon, B., Julian, J., Coego, A., Wu, Q., Zhang, X., Batistic, O., Alquraishi, S. A., Kudla, J., An, C., and Rodriguez, P. L (2019)
    ABA inhibits myristoylation and induces shuttling of the RGLG1 E3 ligase to promote nuclear degradation of PP2CA
    Plant J. 98(5):813-825
  • Pedro L. Rodriguez, Jorge Lozano-Juste and Armando Albert (2019)
    PYR/PYL/RCAR ABA receptors
    Advances in Botanical Research, Abscisic Acid in Plants 92
  • Fernandez, M. A., Belda-Palazon, B., Julian, J., Coego, A., Lozano-Juste, J., Inigo, S., Rodriguez, L., Bueso, E., Goossens, A., and Rodriguez, P. L (2019)
    RBR-type E3 ligases and the Ub-conjugating enzyme UBC26 regulate ABA receptor levels and signaling
    Plant Physiol. DOI:10.1104/pp.19.00898
  • Garcia-Leon, M., Cuyas, L., El-Moneim, D., Rodriguez, L., Belda-Palazon, B., Fernandez, Y., Roux, B., Zamarreño, A.M., Garcia-Mina, J.M., Nussaume, L., Rodriguez, P.L., Paz-Ares, J., Leonhardt, N., and Rubio, V. (2019)
    Stomatal aperture and turnover of ABA receptors are regulated by Arabidopsis ALIX
    Plant Cell 31(10):2411-2429
  • Lozano-Juste, J., Masi, M., Cimmino, A., Clement, S., Fernandez, M. A., Antoni, R., Meyer, S., Rodriguez, P. L., and Evidente, A (2019)
    The fungal sesquiterpenoid pyrenophoric acid B uses the plant ABA biosynthetic pathway to inhibit seed germination
    J.Exp.Bot. 70(19):5487-5494
  • Julian, J., Coego, A., Lozano-Juste, J., Lechner, E., Wu, Q., Zhang, X., Merilo, E., Belda-Palazon, B., Park, S. Y., Cutler, S. R., An, C., Genschik, P., and Rodriguez, P. L (2019)
    The MATH-BTB BPM3 and BPM5 subunits of Cullin3-RING E3 ubiquitin ligases target PP2CA and other clade A PP2Cs for degradation
    Proc.Natl.Acad.Sci.U.S.A. 116(31):15725-15734
  • Li, H., Li, Y., Zhao, Q., Li, T., Wei, J., Li, B., Shen, W., Yang, C., Zeng, Y., Rodriguez, P. L., Zhao, Y., Jiang, L., Wang, X., and Gao, C. (2019)
    The plant ESCRT component FREE1 shuttles to the nucleus to attenuate abscisic acid signalling
    Nature Plants. 5(5):512-524
  • Dittrich, M., Mueller, H. M., Bauer, H., Peirats-Llobet, M., Rodriguez, P. L., Geilfus, C. M., Carpentier, S. C., Al Rasheid, K. A. S., Kollist, H., Merilo, E., Herrmann, J., Muller, T., Ache, P., Hetherington, A. M., and Hedrich, R (2019)
    The role of Arabidopsis ABA receptors from the PYR/PYL/RCAR family in stomatal acclimation and closure signal integration
    Nature Plants 5(9), 1002-1011.
  • Forster, S., Schmidt, L. K., Kopic, E., Anschutz, U., Huang, S., Schlucking, K., Koster, P., Waadt, R., Larrieu, A., Batistic, O., Rodriguez, P. L., Grill, E., Kudla, J., and Becker, D (2019)
    Wounding-induced stomatal closure requires jasmonate-mediated activation of GORK K+ channels by a Ca2+ sensor-kinase CBL1-CIPK5 complex
    Developmental Cell 48(1):87-99.e6
  • Belda-Palazon, B., Gonzalez-Garcia, M. P., Lozano-Juste, J., Coego, A., Antoni, R., Julian, J., Peirats-Llobet, M., Rodriguez, L., Berbel, A., Dietrich, D., Fernandez, M. A., Madueno, F., Bennett, M. J., and Rodriguez, P. L (2018)
    PYL8 mediates ABA perception in the root through non-cell-autonomous and ligand-stabilization-based mechanisms
    Proc.Natl.Acad.Sci. USA 115, E11857-E11863
Forster, S., Schmidt, L. K., Kopic, E., Anschutz, U., Huang, S., Schlucking, K., Koster, P., Waadt, R., Larrieu, A., Batistic, O., Rodriguez, P. L., Grill, E., Kudla, J., and Becker, D (2019)
Wounding-induced stomatal closure requires jasmonate-mediated activation of GORK K+ channels by a Ca2+ sensor-kinase CBL1-CIPK5 complex
Developmental Cell 48(1):87-99.e6.
Belda-Palazon, B., Julian, J., Coego, A., Wu, Q., Zhang, X., Batistic, O., Alquraishi, S. A., Kudla, J., An, C., and Rodriguez, P. L. (2019)
ABA inhibits myristoylation and induces shuttling of the RGLG1 E3 ligase to promote nuclear degradation of PP2CA
Plant J. doi10.1111/tpj.14274
Li, H., Li, Y., Zhao, Q., Li, T., Wei, J., Li, B., Shen, W., Yang, C., Zeng, Y., Rodriguez, P. L., Zhao, Y., Jiang, L., Wang, X., and Gao, C. (2019)
The plant ESCRT component FREE1 shuttles to the nucleus to attenuate abscisic acid signalling.
Nature Plants: https://rdcu.be/bBIGF
Orman-Ligeza B, Morris EC, Parizot B, Lavigne T, Babé A, Ligeza A, Klein S, Sturrock C, Xuan W, Novák O, Ljung K, Fernandez MA, Rodriguez PL, Dodd IC, De Smet I, Chaumont F, Batoko H, Périlleux C, Lynch JP, Bennett MJ, Beeckman T, Draye X. (2018)
Xerobranching Response Represses Lateral Root Formation When Roots Are Not in Contact With Water.
Current Biology 28, 3165-3173.
Belda-Palazon, B., Gonzalez-Garcia, M. P., Lozano-Juste, J., Coego, A., Antoni, R., Julian, J., Peirats-Llobet, M., Rodriguez, L., Berbel, A., Dietrich, D., Fernandez, M. A., Madueno, F., Bennett, M. J., and Rodriguez, P. L. (2018)
PYL8 mediates ABA perception in the root through non-cell-autonomous and ligand-stabilization-based mechanisms
Proc.Natl.Acad.Sci. USA 115, E11857-E11863.
Arbona,V., Zandalinas,S.I., Manzi,M., Gonzalez-Guzman,M., Rodriguez,P.L. and Gomez-Cadenas,A. (2017)
Depletion of abscisic acid levels in roots of flooded Carrizo citrange (Poncirus trifoliata L. Raf. x Citrus sinensis L. Osb.) plants is a stress-specific response associated to the differential expression of PYR/PYL/RCAR receptors
Plant Mol. Biol. 93, 623-640. doi: 10.1007/s11103-017-0587-7
Vicente,J., Mendiondo,G.M., Movahedi,M., Peirats-Llobet,M., Juan,Y.T., Shen,Y.Y., Dambire,C., Smart,K., Rodriguez,P.L., Charng,Y.Y., Gray,J.E. and Holdsworth,M.J. (2017)
The Cys-Arg/N-End Rule Pathway Is a General Sensor of Abiotic Stress in Flowering Plants.
Current Biology 27, 3183-3190
Pizzio, G.A. and Rodriguez, P.L. (2017)
In-gel-kinase assay.
Bioprotocols, Vol 7, 1-12, DOI:10.21769/BioProtoc.2170.
Dietrich,D., Pang,L., Kobayashi,A., Fozard,J.A., Boudolf,V.+., Bhosale,R., Antoni,R., Nguyen,T., Hiratsuka,S., Fujii,N., Miyazawa,Y., Bae,T.W., Wells,D.M., Owen,M.R., Band,L.R., Dyson,R.J., Jensen,O.E., King,J.R., Tracy,S.R., Sturrock,C.J., Mooney,S.J., Roberts,J.A., Bhalerao,R.P., Dinneny,J.R., Rodriguez,P.L., Nagatani,A., Hosokawa,Y., Baskin,T.I., Pridmore,T.P., De Veylder,L., Takahashi,H. and Bennett,M.J. (2017)
Root hydrotropism is controlled via a cortex-specific growth mechanism.
Nature Plants 3, 17057.
Ling,Y., Alshareef,S., Butt,H., Lozano-Juste,J., Li,L., Galal,A.A., Moustafa,A., Momin,A.A., Tashkandi,M., Richardson,D.N., Fujii,H., Arold,S., Rodriguez,P.L., Duque,P. and Mahfouz,M.M. (2017)
Pre-mRNA splicing repression triggers abiotic stress signaling in plants.
Plant Journal 89, 291-309, doi: 10.1111/tpj.13383

DrugCrops. Drug discovery to improve drought tolerance in crops

(707477), 01-01-2017/31-12-2018

Entidad financiadora: 
H2020-MSCA-IF-2015
IP: 
Dr Pedro L Rodriguez, Dr Jorge Lozano.

Regulación de la señalización del ABA y tolerancia a sequía  mediante E3 ubiquitín ligasas que regulan el recambio de receptores y fosfatasas 2C. BIO2017-82503-R

Entidad financiadora: 
MICINN
IP: 
Dr Pedro L. Rodriguez

Descubrimiento de agroquímicos para mejorar la resistencia a la sequía de plantas de cosecha. RETOS colaboración EMPRESA. RTC-2017-6019-2

Entidad financiadora: 
MICINN
IP: 
Dr Pedro L. Rodriguez

Edición de genoma y biología química para aumentar la tolerancia a sequía de los cultivos. EUROPA INVESTIGACION 2017-86741

Entidad financiadora: 
MICINN
IP: 
Dr Pedro L. Rodriguez
Título: 
Plantas resistentes al estrés hídrico y salino y procedimiento para la obtención de las mismas/ Plants resistant to saline and water stress and method for obtaining them
Fecha de solicitud: 
Wednesday, 1 February, 2006
Referencia ID: 
Nº DE SOLICITUD: P200600218 PCT/ES2007/070023
Autores: 
S A. Sáez, R. Serrano y P.L. Rodríguez (p.o. de firma):
Título: 
Utilizacion del enzima fosfopanteteina adeniltrasferasa, implicado en la biosíntesis del coenzima a, en la mejora del crecimiento vegetal, resistencia al estrés salino/osmótico, incremento de lípidos de reserva y modificación del contenido aminoacídico
Fecha de solicitud: 
Monday, 12 May, 2008
Referencia ID: 
P200600218 PCT/ES2007/070023
Autores: 
S. Rubio y P.L. Rodríguez (p.o. de firma)
Título: 
TRANSGENIC PLANTS. Method for manipulation of the ABA signaling pathway and transgenic plants with improved stress resistance.
Fecha de solicitud: 
Monday, 13 May, 2013
Referencia ID: 
European Patent Application No. EP13382177.7
Autores: 
P.L. Rodríguez
Fundamental and applied research in ABA signaling: Regulation by ABA of the chromatin remodeling ATPase BRAHMA and biotechnological use of the PP2CA promoter
Año: 
2017
Nombre: 
Peirats-Llober, Marta
Universidad: 
Universitat Politècnica de Valencia
Dirigida por: 
Pedro L. Rodriguez
Tipo: 
Tesis Doctorales
Identification of Targets and Auxiliary Proteins of PYR/PYL/RCAR ABA receptors: Protein Phosphatase Type 2C (PP2Cs) and C2-Domain ABA-related Proteins (CARs)
Año: 
2015
Nombre: 
Lesia Rodriguez
Universidad: 
Universitat Politecnica de Valencia
Dirigida por: 
Rodríguez, Pedro L.
Tipo: 
Tesis Doctorales
Molecular and genetic analyses of the PP2C-ABA RECEPTOR interaction in the abscisic acid signaling pathway
Año: 
2013
Nombre: 
Regina Antoni
Universidad: 
Universitat Politecnica de Valencia
Dirigida por: 
Rodríguez, Pedro L.
Tipo: 
Tesis Doctorales
Structural insights into ABA perception and signaling: structure of ABA receptor PYR1
Año: 
2011
Nombre: 
Julia Santiago
Universidad: 
Universitat Politecnica de Valencia
Dirigida por: 
Rodríguez, Pedro L.
Tipo: 
Tesis Doctorales
Ruta de transducción de señal del ácido abscísico: Regulación por HAB1 y dianas de interacción. La inactivación combinada de PP2Cs como herramienta biotecnológica para incrementar la tolerancia a sequía en plantas
Año: 
2010
Nombre: 
Angela Saez
Universidad: 
Universitat Politecnica de Valencia
Dirigida por: 
Rodríguez, Pedro L.
Tipo: 
Tesis Doctorales
La biosíntesis del coenzima A y su papel en el establecimiento de la plántula, en la respuesta al estrés salino/osmótico y en el almacenamiento de los lípidos en Arabidopsis thaliana.
Año: 
2009
Nombre: 
Rubio Novella, Silvia
Universidad: 
Universitat Politecnica de Valencia
Dirigida por: 
Rodríguez, Pedro L.
Tipo: 
Tesis Doctorales
Abscisic acid signal transduction: Regulation by HAB1 and interaction with brassinosteroids mediated by BRX
Año: 
2009
Nombre: 
Rodrigues, Américo
Universidad: 
Universitat Politecnica de Valencia
Dirigida por: 
Rodríguez, Pedro L.
Tipo: 
Tesis Doctorales
Modulación de la resistencia a sequía por el receptor de ABA PYL5 mediante la inhibición de proteínas fosfatasa tipo 2C.
Año: 
2009
Nombre: 
Santiago, Julia
Universidad: 
Universitat Politecnica de Valencia, D.E.A.
Dirigida por: 
Rodríguez, Pedro L.
Tipo: 
Tesis de Master
Análisis genético de mutantes hipersensibles al ácido abscísico
Año: 
2008
Nombre: 
Rodrigues, Américo
Universidad: 
Universitat Politecnica de Valencia, D.E.A.
Dirigida por: 
Rodríguez, Pedro L.
Tipo: 
Tesis de Master
Caracterización de mutantes de Arabidopsis afectados en la biosíntesis de Coenzima A y su implicación en el establecimiento de plántula y la respuesta al estrés salino.
Año: 
2008
Nombre: 
Rubio Novella, Silvia
Universidad: 
Universitat Politecnica de Valencia, D.E.A.
Dirigida por: 
Rodríguez, Pedro L.
Tipo: 
Tesis de Master