ID pYFN43 standard; circular DNA; ; 12251 BP. DE pMDC24. KW pMDC24. CC This file is created by Vector NTI CC http://www.informaxinc.com/ CC ORIGDB|GenBank CC VNTDATE|368464372| CC VNTDBDATE|387547121| CC LSOWNER| CC VNTAUTHORNAME|Alejandro| FH Key Location/Qualifiers FH FT CDS complement(8631..9425) FT /vntifkey="4" FT /label=kanamycin\resistance FT misc_feature complement(3591..3616) FT /vntifkey="21" FT /label=RB FT misc_feature 9850..9875 FT /vntifkey="21" FT /label=LB FT CDS complement(10166..11191) FT /vntifkey="4" FT /label=hygromycin\resistance FT rep_origin complement(6250..7250) FT /vntifkey="33" FT /label=pVS1 FT rep_origin complement(8060..8340) FT /vntifkey="33" FT /label=pBR322\origin FT promoter complement(2563..3331) FT /vntifkey="29" FT /label=2X35S\promoter FT terminator 6..263 FT /vntifkey="43" FT /label=nos\terminator FT repeat_region 319..443 FT /vntifkey="34" FT /label=attR2 FT CDS complement(484..789) FT /vntifkey="4" FT /label=ccdB FT CDS complement(1131..1790) FT /vntifkey="4" FT /label=CMr FT repeat_region 1899..2028 FT /vntifkey="34" FT /label=attR1 FT misc_feature complement(2094..2554) FT /vntifkey="21" FT /label=YFN FT misc_feature complement(2042..2093) FT /vntifkey="21" FT /label=FlexibleLinker SQ Sequence 12251 BP; 2903 A; 3273 C; 3166 G; 2909 t; aattcagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt 60 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca 120 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt 180 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa 240 tgtttgaacg atcggggaaa ttcgagctcc accgcggtgg gcggccgctc tagaactagt 300 taattaagga attatcgaac cactttgtac aagaaagctg aacgagaaac gtaaaatgat 360 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa 420 acacaacata tccagtcact atggtcgacc tgcagactgg ctgtgtataa gggagcctga 480 catttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct 540 gagatcagcc acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat 600 catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg agactttatc 660 tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat 720 atcactctgt acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt 780 aaactgcatt tcaccagtcc ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc 840 gggcgacctc agccatccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac 900 gggcttcatt ctgcatggtt gtgcttacca gaccggagat attgacatca tatatgcctt 960 gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcacacct 1020 ctttttgaca tacttcgggt atacatatca gtatatattc ttataccgca aaaatcagcg 1080 cgcaaatacg catactgtta tctggctttt agtaagccgg atcctctaga ttacgccccg 1140 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca 1200 tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta 1260 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa 1320 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac 1380 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt 1440 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc 1500 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc 1560 attgccatac ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc 1620 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga 1680 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga 1740 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc 1800 ttagctcctg aaaatctcgc cggatcctaa ctcaaaatcc acacattata cgagccggaa 1860 gcataaagtg taaagcctgg ggtgcctaat gcggccgcca tagtgactgg atatgttgtg 1920 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta 1980 tatcatttta cgtttctcgt tcagcttttt tgtacaaact tgtttgatag cttggcgcgc 2040 caagagatcc acctccacca gatccacctc caccagatcc acctccaccg gccatgatat 2100 agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct 2160 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct 2220 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc 2280 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga 2340 agcactgcag gccgtagccg aaggtggtca cgagggtggg ccagggcacg ggcagcttgc 2400 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc 2460 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca 2520 ccccggtgaa cagctcctcg cccttgctca ccatggtacc cggggatcct ctagagtcga 2580 ggtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg 2640 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga 2700 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg 2760 accactgtcg gcagaggcat cttcaacgat ggcctttcct ttatcgcaat gatggcattt 2820 gtaggagcca ccttcctttt ccactatctt cacaataaag tgacagatag ctgggcaatg 2880 gaatccgagg aggtttccgg atattaccct ttgttgaaaa gtctcaattg ccctttggtc 2940 ttctgagact gtatctttga tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt 3000 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct 3060 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt caacgatggc 3120 ctttccttta tcgcaatgat ggcatttgta ggagccacct tccttttcca ctatcttcac 3180 aataaagtga cagatagctg ggcaatggaa tccgaggagg tttccggata ttaccctttg 3240 ttgaaaagtc tcaattgccc tttggtcttc tgagactgta tctttgatat ttttggagta 3300 gacaagtgtg tcgtgctcca ccatgttgac ctgcaggcac gccaagcttg gcactggccg 3360 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 3420 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 3480 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg 3540 tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa 3600 gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt 3660 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat 3720 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga 3780 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg 3840 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag 3900 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac 3960 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct 4020 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga 4080 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac 4140 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct 4200 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc 4260 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc 4320 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc 4380 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct 4440 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac 4500 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc 4560 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac 4620 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc 4680 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat 4740 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta 4800 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg 4860 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc 4920 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg 4980 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg 5040 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg 5100 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg 5160 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc 5220 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca 5280 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg 5340 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca 5400 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac 5460 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca 5520 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg 5580 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat 5640 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt 5700 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta 5760 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa 5820 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt 5880 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc 5940 ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg 6000 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag 6060 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc 6120 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa 6180 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg 6240 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac 6300 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg 6360 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg 6420 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag 6480 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg 6540 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg 6600 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg 6660 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc 6720 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc 6780 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc 6840 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg 6900 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag 6960 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt 7020 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa 7080 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg 7140 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact 7200 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact 7260 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa 7320 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc 7380 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga 7440 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg 7500 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 7560 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 7620 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac 7680 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 7740 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 7800 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 7860 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 7920 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 7980 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 8040 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 8100 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 8160 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 8220 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 8280 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 8340 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 8400 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 8460 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 8520 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 8580 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat 8640 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca 8700 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag 8760 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact 8820 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc 8880 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg 8940 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat 9000 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga 9060 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca 9120 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca 9180 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg 9240 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa 9300 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta 9360 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta 9420 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt 9480 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa 9540 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt 9600 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc 9660 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat 9720 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac 9780 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg 9840 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa 9900 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat 9960 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa 10020 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac 10080 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga 10140 tcgacagatc cggtcggcat ctactctatt tctttgccct cggacgagtg ctggggcgtc 10200 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc 10260 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac 10320 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa 10380 gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc 10440 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt 10500 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta 10560 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga 10620 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac 10680 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata 10740 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc 10800 tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa 10860 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga 10920 tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg 10980 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat 11040 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc 11100 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga 11160 cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggga tctgcgaaag 11220 ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga 11280 aatgaacttc cttatataga ggaaggtctt gcgaaggata gtgggattgt gcgtcatccc 11340 ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt 11400 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg 11460 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct 11520 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc 11580 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt 11640 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttatcacat caatccactt 11700 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc 11760 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat 11820 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag 11880 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag 11940 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct 12000 ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg cctctccccg cgcgttggcc 12060 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 12120 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 12180 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 12240 ccatgattac g 12251 //