• Juarez P, Virdi V, Depicker A, Orzaez D. (2016) Biomanufacturing of protective antibodies and other therapeutics in edible plant tissues for oral applications. Plant Biotechnol J. DOI: 10.1111/pbi.12541
  • Dicker M, Tschofen M, Maresch D, König J, Juarez P, Orzaez D, Altmann F, Steinkellner H, Strasser R. (2016) Transient Glyco-Engineering to Produce Recombinant IgA1 with Defined N- and O-Glycans in Plants. Front Plant Sci. 7:18. doi: 10.3389/fpls.2016.00018.
  • Vazquez-Vilar M, Bernabé-Orts JM, Fernandez-Del-Carmen A, Ziarsolo P, Blanca J, Granell A, Orzaez D. (2016) A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods 12:10 DOI: 10.1186/s13007-016-0101-2
  • Polturak G, Breitel D, Grossman N, Sarrion-Perdigones A, Weithorn E, Pliner M, Orzaez D, Granell A, Rogachev I, Aharoni A. (2016) Elucidation of the first committed step in betalain biosynthesis enables the heterologous engineering of betalain pigments in plants. New Phytol. 210(1):269-283


  • Lashbrooke J, Adato A, Lotan O, Alkan N, Tsimbalist T, Rechav K, Fernandez-Moreno JP, Widemann E, Grausem B, Pinot F, Granell A, Costa F, Aharoni A.(2015) The Tomato MIXTA-Like Transcription Factor Coordinates Fruit Epidermis Conical Cell Development and Cuticular Lipid Biosynthesis and Assembly. Plant Physiol 169(4):2553-71
  • Patron NJ, Orzaez D, Marillonnet S, Warzecha H, Matthewman C, Youles M, Raitskin O, Leveau A, Farré G, Rogers C, Smith A, Hibberd J, Webb AA, Locke J, Schornack S, Ajioka J, Baulcombe DC, Zipfel C, Kamoun S, Jones JD, Kuhn H, Robatzek S, Van Esse HP, Sanders D, Oldroyd G, Martin C, Field R, O'Connor S, Fox S, Wulff B, Miller B, Breakspear A, Radhakrishnan G, Delaux PM, Loqué D, Granell A, Tissier A, Shih P, Brutnell TP, Quick WP, Rischer H, Fraser PD, Aharoni A, Raines C, South PF, Ané JM, Hamberger BR, Langdale J, Stougaard J, Bouwmeester H, Udvardi M, Murray JA, Ntoukakis V, Schäfer P, Denby K, Edwards KJ, Osbourn A, Haseloff J (2015) Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytol. 208(1):13-9
  • Capel C, Fernández Del Carmen A, Alba JM, Lima-Silva V, Hernández-Gras F, Salinas M, Boronat A, Angosto T, Botella MA, Fernández-Muñoz R, Granell A, Capel J, Lozano R. (2015) Wide-genome QTL mapping of fruit quality traits in a tomato RIL population derived from the wild-relative species Solanum pimpinellifolium L. Theor Appl Genet. 128(10):2019-2035
  • Puig CP, Dagar A, Marti Ibanez C, Singh V, Crisosto CH, Friedman H, Lurie S, Granell A. (2015) Pre-symptomatic transcriptome changes during cold storage of chilling sensitive and resistant peach cultivars to elucidate chilling injury mechanisms. BMC Genomics 16(1):245
  • Rutten V, Munabi A, Riche F, Lewy G, Wilson H, Pipan M, Bhate S, Nghiem T, Kaufhold W, Haselhoff J, Rubert A, Gonzalez A, Quijano A, Llopis I, Gavalda J, Estelles L, Vazquez M, Orzaez D, Deal C, Gray J, Spiegel M, Monsey S, Middlemiss A, Day J, Patron N. (2015) A Standard Type IIS Syntax for Plants. BBF RFC 106 doi: 1721.1/96069
  • Vazquez-Vilar M, Sarrion-Perdigones A, Ziarsolo P, Blanca J, Granell A, Orzaez D. (2015) Software-Assisted Stacking of Gene Modules Using GoldenBraid 2.0 DNA-Assembly Framework. Methods Mol Biol 1284:399-420
  • Rambla JL, López-Gresa MP, Bellés JM, Granell A. (2015) Metabolomic profiling of plant tissues. Methods Mol Biol 1284:221-35


  • Barrantes W, Fernández-del-Carmen A, López-Casado G, González-Sánchez MA, Fernández-Muñoz R, Granell A, Monforte AJ (2014) Highly efficient genomics-assisted development of a library of introgression lines of Solanum pimpinellifolium. Mol Breeding 34(4):1817-31
  • Seymour GB, Granell A. (2014) Fruit development and ripening. J Exp Bot 65(16):4489-90
  • Rubio-Moraga A, Rambla JL, Fernández-de-Carmen A, Trapero-Mozos A, Ahrazem O, Orzáez D, Granell A, Gómez-Gómez L.(2014) New target carotenoids for CCD4 enzymes are revealed with the characterization of a novel stress-induced carotenoid cleavage dioxygenase gene from Crocus sativus. Plant Mol Biol 86(4-5):555-69
  • Renau-Morata B, Sánchez-Perales M, Medina J, Molina RV, Corrales R, Carrillo L, Fernández-Nohales P, Marqués J, Pollmann S, Vicente-Carbajosa J, Granell A, Nebauer, SG. (2014) Salinity Assay in Tomato. Bio-protocol 4(16): e1215
  • Corrales AR, Carrillo L, Nebauer SG, Renau-Morata B, Sánchez-Perales M, Fernández-Nohales P, Marqués J, Granell A, Pollmann S, Vicente-Carbajosa J, Molina RV, Medina J. (2014) Salinity Assay in Arabidopsis. Bio-protocol 4(16): e1216
  • Juarez P, Fernandez-Del-Carmen A, Rambla JL, Presa S, Mico A, Granell A, Orzaez D. (2014) Evaluation of Unintended Effects in the Composition of Tomatoes Expressing a Human Immunoglobulin A against Rotavirus. J Agric Food Chem 62(32):8158-68
  • Belda-Palazón B, Nohales MA, Rambla JL, Aceña JL, Delgado O, Fustero S, Martínez MC, Granell A, Carbonell J, Ferrando A. (2014) Biochemical quantitation of the eIF5A hypusination in Arabidopsis thaliana uncovers ABA-dependent regulation. Front Plant Sci 5:202
  • Sánchez G, Martínez J, Romeu J, García J, Monforte AJ, Badenes ML, Granell A. (2014) The peach volatilome modularity is reflected at the genetic and environmental response levels in a QTL mapping population. BMC Plant Biol 14:137
  • Cocaliadis MF, Fernández-Muñoz R, Pons C, Orzaez D, Granell A. (2014) Increasing tomato fruit quality by enhancing fruit chloroplast function. A double-edged sword? J Exp Bot 65(16):4589-98
  • González-Guzmán M, Rodríguez L, Lorenzo-Orts L, Pons C, Sarrión-Perdigones A, Fernández MA, Peirats-Llobet M, Forment J, Moreno-Alvero M, Cutler SR, Albert A, Granell A, Rodríguez PL. (2014) Tomato PYR/PYL/RCAR abscisic acid receptors show high expression in root, differential sensitivity to the abscisic acid agonist quinabactin, and the capability to enhance plant drought resistance. J Exp Bot 65(15):4451-64
  • Rambla JL, Tikunov YM, Monforte AJ, Bovy AG, Granell A. (2014) The expanded tomato fruit volatile landscape. J Exp Bot [Epub ahead of print, doi: 10.1093/jxb/eru128]
  • Pons C, Martí C, Forment J, Crisosto CH, Dandekar AM, Granell A. (2014) A bulk segregant gene expression analysis of a peach population reveals components of the underlying mechanism of the fruit cold response. PLoS One 9(3):e90706
  • Romeu JF, Monforte AJ, Sánchez G, Granell A, García-Brunton J, Badenes ML, Ríos G. (2014) Quantitative trait loci affecting reproductive phenology in peach. BMC Plant Biol 14(1):52
  • Corrales AR, Nebauer SG, Carrillo L, Fernández-Nohales P, Marqués J, Renau-Morata B, Granell A, Pollmann S, Vicente-Carbajosa J, Molina RV, Medina J. (2014) Characterization of tomato Cycling Dof Factors reveals conserved and new functions in the control of flowering time and abiotic stress responses. J Exp Bot 65(4):995-1012
  • Sarrion-Perdigones A, Palaci J, Granell A, Orzaez D. (2014) Design and construction of multigenic constructs for plant biotechnology using the GoldenBraid cloning strategy. Methods Mol Biol 1116:133-51


  • Rubio Moraga A, Ahrazem O, Rambla JL, Granell A, Gómez Gómez L. (2013) Crocins with high levels of sugar conjugation contribute to the yellow colours of early-spring flowering crocus tepals. PLoS One 8(9):e71946
  • Aragüez I, Osorio S, Hoffmann T, Rambla JL, Medina-Escobar N, Granell A, Botella MÁ, Schwab W, Valpuesta V. (2013) Eugenol production in achenes and receptacles of strawberry fruits is catalyzed by synthases exhibiting distinct kinetics. Plant Physiol 163(2):946-58
  • Tikunov YM, Molthoff J, de Vos RC, Beekwilder J, van Houwelingen A, van der Hooft JJ, Nijenhuis-de Vries M, Labrie CW, Verkerke W, van de Geest H, Viquez Zamora M, Presa S, Rambla JL, Granell A, Hall RD, Bovy AG. (2013) NON-smoky glycosyltransferase1 prevents the release of smoky aroma from tomato fruit. Plant Cell 25(8): 3067-3078
  • Herranz MC, Niehl A, Rosales M, Fiore N, Zamorano A, Granell A, Pallas V. (2013) A remarkable synergistic effect at the transcriptomic level in peach fruits doubly infected by prunus necrotic ringspot virus and peach latent mosaic viroid. Virol J 10:164-178.
  • Zhang Y, Butelli E, De Stefano R, Schoonbeek HJ, Magusin A, Pagliarani C, Wellner N, Hill L, Orzaez D, Granell A, Jones JD, Martin C. (2013) Anthocyanins Double the Shelf Life of Tomatoes by Delaying Overripening and Reducing Susceptibility to Gray Mold. Curr Biol 23(12):1094-100
  • Sánchez G, Venegas-Calerón M, Salas JJ, Monforte A, Badenes ML, Granell A. (2013) An integrative "omics" approach identifies new candidate genes to impact aroma volatiles in peach fruit. BMC Genomics 14: 343-365
  • Sarrion-Perdigones A, Vazquez-Vilar M, Palací J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D. (2013) GoldenBraid2.0: A comprehensive DNA assembly framework for Plant Synthetic Biology. Plant Physiol 162(3):1618-31
  • Estornell LH, Pons C, Martínez A, O'Connor JE, Orzaez D, Granell A. (2013) A VIN1 GUS::GFP fusion reveals activated sucrose metabolism programming occurring in interspersed cells during tomato fruit ripening. J Plant Physiol 170(12):1113-21
  • Juarez P, Huet-Trujillo E, Sarrion-Perdigones A, Falconi EE, Granell A, Orzaez D. (2013) Combinatorial Analysis of Secretory Immunoglobulin A (sIgA) Expression in Plants. Int J Mol Sci 14(3): 6205-6222
  • Julve JM, Gandía A, Fernández-Del-Carmen A, Sarrion-Perdigones A, Castelijns B, Granell A, Orzaez D. (2013) A coat-independent superinfection exclusion rapidly imposed in Nicotiana benthamiana cells by tobacco mosaic virus is not prevented by depletion of the movement protein. Plant Mol Biol 81(6):553-64
  • Fernandez-Del-Carmen A, Juárez P, Presa S, Granell A, Orzáez D. (2013) Recombinant jacalin-like plant lectins are produced at high levels in Nicotiana benthamiana and retain agglutination activity and sugar specificity. J Biotechnol 163(4): 391-400
  • Fernandez-Moreno JP, Orzaez D, Granell A. (2013) VIGS: A Tool to Study Fruit Development in Solanum lycopersicum. Methods Mol Biol 975: 183-96
  • Shi JX, Adato A, Alkan N, He Y, Lashbrooke J, Matas AJ, Meir S, Malitsky S, Isaacson T, Prusky D, Leshkowitz D, Schreiber L, Granell AR, Widemann E, Grausem B, Pinot F, Rose JK, Rogachev I, Rothan C, Aharoni A. (2013) The tomato SlSHINE3 transcription factor regulates fruit cuticle formation and epidermal patterning. New Phytol 197(2): 468-80


  • Altosaar I, Oehmen A, Almeida CM, De Pascale S, de Tommasi N, Domínguez de María P, Orzaez D, Zervos A, Xu C. (2012) Funding decisions: Romania needs overseas reviewers. Nature 492(7428): 186
  • Mounet F, Moing A, Kowalczyk M, Rohrmann J, Petit J, Garcia V, Maucourt M, Yano K, Deborde C, Aoki K, Berges H, Granell A, Fernie AR, Bellini C, Rothan C, Lemaire-Chamley M. (2012) Down-regulation of a single auxin efflux transport protein in tomato induces precocious fruit development. J Exp Bot 63(13): 4901-4917
  • Powell A L, Nguyen CV, Hill T, Cheng KL, Figueroa-Balderas R, Aktas H, Ashrafi H, Pons C, Fernandez-Munoz R, Vicente A, Lopez-Baltazar J, Barry CS, Liu Y, Chetelat R, Granell A, Van Deynze A, Giovannoni JJ, Bennett AB. (2012) Uniform ripening encodes a Golden 2-like transcription factor regulating tomato fruit chloroplast development. Science 336(6089): 1711-1715
  • Sanchez G, Besada C, Badenes ML, Monforte AJ, Granell A. (2012) A non-targeted approach unravels the volatile network in peach fruit. PloSOne 7(6): e38992
  • Carrera J, Fernández-del-Carmen A, Fernández-Muñoz R, Rambla JL, Pons C, Jaramillo A, Elena SF, Granell A. (2012) Fine-tuning tomato agronomic properties by computational genome redesign. PLoS Comput Biol 8(6): e1002528
  • Tomato Genome Consortium (2012) The tomato genome sequence provides insights into fleshy fruit evolution. Nature 485(7400): 635-41
  • Lima-Silva V, Rosado A, Amorin-Silva V, Muñoz-Mérida A, Pons C, Bombarely A, Trelles O, Fernández-Muñoz R, Granell A, Valpuesta V Botella MA. (2012) Genetic and genome-wide transcriptomic analyses identify co-regulation of oxidative response and hormone transcript abundance with vitamin c content in tomato fruit. BMC Genomics 13(1): 187
  • Zorrilla-Fontanesi Y, Rambla JL, Cabeza A, Medina JJ, Sánchez-Sevilla JF, Valpuesta V, Botella MA, Granell A, Amaya I. (2012) Genetic Analysis of Strawberry Fruit Aroma and Identification of O-Methyltransferase FaOMT as the Locus Controlling Natural Variation in Mesifurane Content. Plant Physiol 159(2): 851-870
  • Martin C, Zhang Y, Tomlinson L, Kallam K, Luo J, Jones JDG, Granell A, Orzaez D, Butelli E. (2012) Colouring up Plant Biotechnology. Recent Advances in Polyphenol Research Volume 3 (eds V. Cheynier, P. Sarni-Manchado and S. Quideau), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9781118299753.ch5
  • Estornell LH, Granell A, Orzáez D. (2012) Exploiting multisite Gateway and pENFRUIT plasmid collection for fruit genetic engineering. Methods Mol Biol 847: 351-68
  • Bedoya LC, Martínez F, Orzáez D, Darós JA. (2012) Visual tracking of plant virus infection and movement using a reporter MYB transcription factor that activates anthocyanin biosynthesis. Plant Physiol 158(3): 1130-8
  • Juárez P, Presa S, Espí J, Pineda B, Antón MT, Moreno V, Buesa J, Granell A, Orzaez D. (2012) Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes. Plant Biotechnol J 10(3): 341-52


  • Lytovchenko A, Eickmeier I, Pons C, Osorio S, Szecowka M, Lehmberg K, Arrivault S, Tohge T, Penida B, Anton MT, Hedtke B, Lu Y, Fisahn J, Bock R, Stitt M, Grimm B, Granell A, Fernie AR. (2011) Tomato fruit photosynthesis is seemingly unimportant in primary metabolism and ripening but plays a considerable role in seed development. Plant Physiol. 157(4): 1650-63
  • González-Mas MC, Rambla JL, Alamar MC, Gutiérrez A, Granell A. (2011) Comparative Analysis of the Volatile Fraction of Fruit Juice from Different Citrus Species. PLoSOne 6(7): e22016. doi:10.1371/journal.pone.0022016
  • Sarrion-Perdigones A, Falconi EE, Zandalinas SI, Juárez P, Fernández-del-Carmen A, Granell A, Orzaez D. (2011) GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules. PLoS One 6(7): e21622. doi:10.1371/journal.pone.0021622
  • Carbonell-Bejerano P, Urbez C, Granell A, Carbonell J, Perez-Amador MA. (2011) Ethylene is involved in pistil fate by modulating the onset of ovule senescence and the GA-mediated fruit set in Arabidopsis. BMC Plant Biol 11: 84.


  • Granell A, Fernández del-Carmen A, Orzáez D. (2010) In planta production of plant-derived and non-plant-derived adjuvants. Expert Rev Vaccines 9(8): 843-58.
  • Carbonell-Bejerano P, Urbez C, Carbonell J, Granell A, Perez-Amador MA. (2010) A fertilization-independent developmental program triggers partial fruit development and senescence processes in pistils of Arabidopsis. Plant Physiol 154(1): 163-72.
  • Orzaez D, Monforte AJ, Granell A. (2010) Using genetic variability available in the breeder's pool to engineer fruit quality. GM Crops 1(3): 120-7.
  • Ballester AR, Molthoff J, de Vos R, Hekkert BL, Orzaez D, Fernández-Moreno JP, Tripodi P, Grandillo S, Martin C, Heldens J, Ykema M, Granell A, Bovy A. (2010) Biochemical and molecular analysis of pink tomatoes: deregulated expression of the gene encoding transcription factor SlMYB12 leads to pink tomato fruit color. Plant Physiol 152(1): 71-84.
  • Rambla JL, Vera-Sirera F, Blázquez MA, Carbonell J, Granell A. (2010) Quantitation of biogenic tetraamines in Arabidopsis thaliana. Anal Biochem 397(2): 208-11.


  • Orzáez D, Granell A, Blázquez MA. (2009) Manufacturing antibodies in the plant cell. Biotechnol J 4(12): 1712-24.
  • Orzaez D, Granell A. (2009) Reverse genetics and transient gene expression in fleshy fruits: overcoming plant stable transformation. Plant Signal Behav 4(9): 864-7.
  • Toronto International Data Release Workshop Authors, Birney E, Hudson TJ, Green ED, Gunter C, Eddy S, Rogers J, Harris JR, Ehrlich SD, Apweiler R, Austin CP, Berglund L, Bobrow M, Bountra C, Brookes AJ, Cambon-Thomsen A, Carter NP, Chisholm RL, Contreras JL, Cooke RM, Crosby WL, Dewar K, Durbin R, Dyke SO, Ecker JR, El Emam K, Feuk L, Gabriel SB, Gallacher J, Gelbart WM, Granell A, Guarner F, Hubbard T, Jackson SA, Jennings JL, Joly Y, Jones SM, Kaye J, Kennedy KL, Knoppers BM, Kyrpides NC, Lowrance WW, Luo J, MacKay JJ, Martín-Rivera L, McCombie WR, McPherson JD, Miller L, Miller W, Moerman D, Mooser V, Morton CC, Ostell JM, Ouellette BF, Parkhill J, Raina PS, Rawlings C, Scherer SE, Scherer SW, Schofield PN, Sensen CW, Stodden VC, Sussman MR, Tanaka T, Thornton J, Tsunoda T, Valle D, Vuorio EI, Walker NM, Wallace S, Weinstock G, Whitman WB, Worley KC, Wu C, Wu J, Yu J. (2009) Prepublication data sharing. Nature 461(7261): 168-70.
  • Moraga AR, Rambla JL, Ahrazem O, Granell A, Gómez-Gómez L. (2009) Metabolite and target transcript analyses during Crocus sativus stigma development. Phytochemistry 70(8): 1009-16.
  • Orzaez D, Medina A, Torre S, Fernández-Moreno JP, Rambla JL, Fernández-Del-Carmen A, Butelli E, Martin C, Granell A. (2009) A visual reporter system for virus-induced gene silencing in tomato fruit based on anthocyanin accumulation. Plant Physiol 150(3): 1122-34.
  • Zanor MI, Rambla JL, Chaïb J, Steppa A, Medina A, Granell A, Fernie AR, Causse M. (2009) Metabolic characterization of loci affecting sensory attributes in tomato allows an assessment of the influence of the levels of primary metabolites and volatile organic contents. J Exp Bot 60(7): 2139-54.
  • Estornell LH, Orzáez D, López-Peña L, Pineda B, Antón MT, Moreno V, Granell A. (2009) A multisite gateway-based toolkit for targeted gene expression and hairpin RNA silencing in tomato fruits. Plant Biotechnol J 7(3): 298-309.


  • Ogundiwin EA, Martí C, Forment J, Pons C, Granell A, Gradziel TM, Peace CP, Crisosto CH. (2008) Development of ChillPeach genomic tools and identification of cold-responsive genes in peach fruit. Plant Mol Biol 68(4-5): 379-97.
  • Rubio A, Rambla JL, Santaella M, Gómez MD, Orzaez D, Granell A, Gómez-Gómez L. (2008) Cytosolic and plastoglobule-targeted carotenoid dioxygenases from Crocus sativus are both involved in beta-ionone release. J Biol Chem 283(36): 24816-25.


  • Martí C, Orzáez D, Ellul P, Moreno V, Carbonell J, Granell A. (2007) Silencing of DELLA induces facultative parthenocarpy in tomato fruits. Plant J 52(5): 865-76.


  • Wieland WH, Lammers A, Schots A, Orzáez DV. (2006) Plant expression of chicken secretory antibodies derived from combinatorial libraries. J Biotechnol 122(3): 382-91.
  • Orzaez D, Mirabel S, Wieland WH, Granell A. (2006) Agroinjection of tomato fruits. A tool for rapid functional analysis of transgenes directly in fruit. Plant Physiol 140(1): 3-11.
  • Wieland WH, Orzáez D, Lammers A, Parmentier HK, Schots A. (2006) Display and selection of chicken IgA Fab fragments. Vet Immunol Immunopathol 110(1-2): 129-40.